Regulation of the SOS response and homologous recombination by an integrative and conjugative element
Garcia Heredia, A.; Grossman, A. D.
Show abstract
Integrative and conjugative elements (ICEs) are mobile genetic elements that transfer between bacteria and influence host physiology and promote evolution. ICEBs1 of Bacillus subtilis modulates the host DNA damage response by reducing RecA filament formation. We found that the two ICEBs1-encoded proteins, RamT and RamA that modulate the SOS response in donors also function in recipient cells to inhibit both the SOS response and homologous recombination following transfer of the element. Expression of RamT and RamA caused a decrease in binding of the host single strand binding protein SsbA to ssDNA. We found that RamA interacted with PcrA, the host DNA translocase that functions to remove RecA from DNA, likely functioning to modulate the SOS response and recombination by stimulating PcrA activity. These findings reveal how ICEBs1 can modulate key host processes, including the SOS response and homologous recombination, highlighting the complex interplay between mobile genetic elements and their bacterial hosts in adaptation and evolution.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- A CRISPR interference screen reveals a role for cell wall teichoic acids in conjugation in Bacillus subtilis 98%
- TnSmu1 is a functional integrative and conjugative element in Streptococcus mutans that when expressed causes growth arrest of host bacteria 97%
- Bacteriophage Lambda RexA and RexB Functions Assist the Transition from Lysogeny to Lytic Growth 95%
Similar papers in this journal
- Biology and engineering of integrative and conjugative elements: Construction and analyses of hybrid ICEs reveal element functions that affect species-specific efficiencies 97%
- Timing of integration into the chromosome is critical for the fitness of an integrative and conjugative element and its bacterial host 95%
- Kar4, the Yeast Homolog of METTL14, is Required for mRNA m6A Methylation and Meiosis 95%
Similar papers in this journal
Similar papers in this journal
- Cassette recruitment in the chromosomal Integron of Vibrio cholerae 95%
- Orchestrated long-distance gene activation by a ParB-like BisD-CTP DNA clamp in low-frequency transfer competence development in Pseudomonas putida 95%
- TlyA is a 23S and 16S 2'-O-methylcytidine methyltransferase important for ribosome assembly in Bacillus subtilis 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.