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The Curated Cancer Cell Atlas: comprehensive characterisation of tumours at single-cell resolution

Tyler, M.; Gavish, A.; Barbolin, C.; Tschernichovsky, R.; Hoefflin, R.; Mints, M.; Puram, S. V.; Tirosh, I.

2024-10-12 cancer biology
10.1101/2024.10.11.617836 bioRxiv
Show abstract

Single-cell RNA-seq (scRNA-seq) has transformed the study of cancer biology. Recent years have seen a rapid expansion in the number of single-cell cancer studies, yet most of these studies profiled few tumours, such that individual datasets have limited statistical power. Combining the data and results across studies holds great promise but also involves various challenges. We recently began to address these challenges by curating a large collection of cancer scRNA-seq datasets, and leveraging it for systematic analyses of tumor heterogeneity. Here we significantly extend this repository to 124 datasets for over 40 cancer types, together comprising 2,822 samples, with improved data annotations, visualisations and exploration. Utilising this vast cohort, we systematically quantified context-dependent gene expression and proliferation patterns across cell types and cancer types. These data, annotations and analysis results are all freely available for exploration and download via the Curated Cancer Cell Atlas (3CA) website (https://www.weizmann.ac.il/sites/3CA/), a central source of data and analyses for the cancer research community that opens new avenues in cancer research.

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