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ALG-1, a microRNA argonaute, promotes vulva induction in C. elegans

Zhen, S.; Rocheleau, C. E.

2024-10-10 developmental biology
10.1101/2024.09.29.615693 bioRxiv
Show abstract

Signaling by the LET-60 Ras GTPase/MPK-1 Extracellular Regulated Kinase pathway specifies the vulva cell fate in C. elegans. The let-7 miRNA family negatively regulates LET-60 Ras but other miRNAs can also modulate vulva induction. To determine the impact of globally reducing miRNA function on LET-60 Ras-mediated vulva induction we analyzed the effect of loss of the ALG-1 miRNA regulator on vulva development. Contrary to our expectations, we find that ALG-1 promotes vulva induction independently of LET-60 Ras. We found that the reduced vulva cell fate induction of alg-1 deletion mutants could be due to delayed development of the vulva, or a requirement to maintain the competence of the uninduced precursor cells.

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