Recurrent introgression and geographical stratification shape Saccharomyces cerevisiae in the Neotropics

HighlightsO_LIPhylogenetic analyses of S. cerevisiae isolates from agave fermentation reveal novel clades within the Neotropical cluster. C_LIO_LIThe Neotropical cluster shows geographical population structure. C_LIO_LIMultiple pulses of interspecific introgression are part of the evolutionary history of S. cerevisiae in the Neotropics C_LI From yeasts to humans, introgressive hybridization significantly influences the evolutionary history of living organisms by introducing new genetic diversity. Strains of Saccharomyces cerevisiae worldwide exhibit introgressions from the sister species S. paradoxus, despite the average sequence identity between these species being lower than 90%. While S. cerevisiae isolates from the Neotropics are known for their high levels of introgression, the evolutionary events leading to the unusually high prevalence of them remain unclear. Here, we sequenced 216 S. cerevisiae isolates living in open agave fermentation across Mexico, a habitat at the interface of natural and industrial environments. The genomes of these strains revealed considerable genetic diversity and population structure linked to geographic distribution, which had been overlooked due to undersampling of this megadiverse region. These strains, along with those from French Guiana, Ecuador, and Brazil, form a broader Neotropical phylogenetic cluster that is notably enriched in introgressed DNA. Surprisingly, the origins and conservation patterns of introgressions indicate multiple hybridization events, suggesting an unprecedented scenario of flexible species barriers in this region. Our findings underscore concurrent evolutionary processes--geographical stratification and multiple introgressions--that shape the genomes of a diverse lineage of S. cerevisiae. Neotropical yeasts thus provide a natural laboratory for exploring the mechanisms and adaptive significance of introgressive hybridization in eukaryotic genome evolution.