Penicillin-binding proteins exhibit catalytic redundancy during asymmetric cell division in Clostridioides difficile

Peptidoglycan synthesis is an essential driver of bacterial growth and division. The final steps of this crucial process involve the activity of the SEDS family glycosyltransferases that polymerize glycan strands and the class B penicillin-binding protein (bPBP) transpeptidases that cross-link them. While many bacteria encode multiple bPBPs to perform specialized roles during specific cellular processes, some bPBPs can play redundant roles that are important for resistance against certain cell wall stresses. Our understanding of these compensatory mechanisms, however, remains incomplete. Endospore-forming bacteria typically encode multiple bPBPs that drive morphological changes required for sporulation. The sporulation-specific bPBP, SpoVD, is important for synthesizing the asymmetric division septum and spore cortex peptidoglycan during sporulation in the pathogen Clostridioides difficile. Although SpoVD catalytic activity is essential for cortex synthesis, we show that it is unexpectedly dispensable for SpoVD to mediate asymmetric division. The dispensability of SpoVDs catalytic activity requires the presence of its SEDS partner, SpoVE, and is facilitated by another sporulation-induced bPBP, PBP3. Our data further suggest that PBP3 interacts with components of the asymmetric division machinery, including SpoVD. These findings suggest a possible mechanism by which bPBPs can be functionally redundant in diverse bacteria and facilitate antibiotic resistance. ImportancePeptidoglycan synthesis requires the transpeptidase activity of penicillin-binding proteins (PBPs), which can have specialized functions during cellular growth, division, and differentiation. However, many bacteria produce PBPs with overlapping functions, and this functional redundancy can increase antibiotic resistance. The spore-forming pathogen, Clostridioides difficile, encodes four PBPs: two are essential for growth and division and another, SpoVD, is essential for spore formation. Here, we show that the transpeptidase activity of SpoVD is dispensable during the first morphological step of sporulation, asymmetric division, because a sporulation-induced PBP, PBP3, partially substitutes for SpoVDs function during this early stage. We find that SpoVD and PBP3 interact during sporulation, suggesting a mechanism by which bPBPs can confer functional redundancy and potentially contribute to antibiotic resistance.