Heterogeneous Kinetics of Nanobubble Ultrasound Contrast Agent and Angiogenic Signaling in Head and Neck Cancer
Van Court, B.; Ciccaglione, M.; Neupert, B.; Knitz, M. W.; Maroney, S. P.; Nguyen, D.; Abdelazeem, K. N. M.; Exner, A. A.; Saviola, A. J.; Benninger, R. K. P.; Karam, S. D.
Show abstract
Recently developed nanobubble ultrasound contrast agents are a promising tool for imaging and drug delivery in tumors. To better understand their unusual kinetics, we implemented a novel pixel clustering analysis, which provides unique information by accounting for spatial heterogeneity. By combining ultrasound results with proteomics of the imaged tumors, we show that this analysis is highly predictive of protein expression and that specific types of nanobubble time-intensity curve are associated with upregulation of different metabolic pathways. We applied this method to study the effects of two proteins, EphB4 and ephrinB2, which control tumor angiogenesis through bidirectional juxtacrine signaling, in mouse models of head and neck cancer. We show that ephrinB2 expression by endothelial cells and EphB4 expression by cancer cells have similar effects on tumor vasculature, despite sometimes opposite effects on tumor growth. This implicates a cancer-cell-intrinsic effect of EphB4 forward signaling and not angiogenesis in EphB4s action as a tumor suppressor.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- High-resolution positron emission microscopy of patient-derived tumor organoids 95%
- Priming versus propagating: distinct immune effects of an alpha- versus beta-particle emitting radiopharmaceutical when combined with immune checkpoint inhibition 95%
- Cellular-level phenotyping of tumor-immune microenvironment (TiME) in patients in vivo reveals distinct inflammation and endothelial anergy signatures 95%
Similar papers in this journal
- Single-Cell Spatial Proteomics Analyses of Head and Neck Squamous Cell Carcinoma Reveal Tumor Heterogeneity and Immune Architectures Associated with Clinical Outcome 95%
- First-in-human prospective trial of sonobiopsy in glioblastoma patients using neuronavigation-guided focused ultrasound 94%
- A Deep Learning Model for Molecular Label Transfer that Enables Cancer Cell Identification from Histopathology Images 93%
Similar papers in this journal
- Spatiotemporal profiling defines persistence and resistance dynamics during targeted treatment of melanoma 94%
- Engineering Tumor Stroma Morphogenesis Using Dynamic Cell-Matrix Spheroid Assembly 94%
- Spatial profiling identifies regionally distinct microenvironments and targetable immunosuppressive mechanisms in pediatric osteosarcoma pulmonary metastases. 93%
Similar papers in this journal
- In vivo optical metabolic imaging of long-chain fatty acid uptake in orthotopic models of triple negative breast cancer 94%
- Propagated circulating tumor cells uncovers the rople of NFκB and COP1 in metastasis 94%
- GABA(A) receptor activation drives GABARAP-Nix mediated autophagy to radiation-sensitize primary and brain-metastatic lung adenocarcinoma tumors 93%
Similar papers in this journal
- Imaging CRISPR-Edited CAR-T Cell Therapies with Optical and Positron Emission Tomography Reporters 94%
- Vascularized tumor on a microfluidic chip to study mechanisms promoting tumor neovascularization and vascular targeted therapies 93%
- Focused ultrasound ablation of melanoma with boiling histotripsy yields abscopal tumor control and antigen-dependent dendritic cell activation 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.