Organellular imaging in vivo reveals a depletion of endoplasmic reticular calcium during post-ictal cortical spreading depolarization
Stern, M. A.; Cole, E. R.; Gutekunst, C.-A.; Yang, J. J.; Berglund, K.; Gross, R. E.
Show abstract
AO_SCPLOWBSTRACTC_SCPLOWDuring cortical spreading depolarization (CSD), neurons exhibit a dramatic increase in cytosolic calcium, which may be integral to CSD-mediated seizure termination. This calcium increase greatly exceeds that during seizures, suggesting the calcium source may not be solely extracellular. Thus, we sought to determine if the endoplasmic reticulum (ER), the largest intracellular calcium store, is involved. We developed a two-photon calcium imaging paradigm to simultaneously record the cytosol and ER during seizures in awake mice. Paired with direct current recording, we reveal that CSD can manifest as a slow post-ictal cytosolic calcium wave with a concomitant depletion of ER calcium that is spatiotemporally consistent with a calcium-induced calcium release. Importantly, we observed both naturally occurring and electrically induced CSD suppressed post-ictal epileptiform activity. Collectively, this work links ER dynamics to CSD, which serves as an innate process for seizure suppression and a potential mechanism underlying therapeutic electrical stimulation for epilepsy.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Extracellular glutamate and GABA transients at the transition from interictal spiking to seizures 98%
- Kcnq2/Kv7.2 controls the threshold and bihemispheric symmetry of cortical spreading depolarization 97%
- Single Unit Analysis and Wide-Field Imaging Reveal Alterations in Excitatory andInhibitory Neurons in Glioma 97%
Similar papers in this journal
- Transient targeting of hypothalamic orexin neurons alleviates seizures in a mouse model of epilepsy 97%
- Multisensory Flicker Modulates Widespread Brain Networks and Reduces Interictal Epileptiform Discharges in Humans 97%
- Developmental Divergence of Sensory Stimulus Representation in Cortical Interneurons 95%
Similar papers in this journal
- Myelin speeds cortical oscillations by consolidating phasic parvalbumin-mediated inhibition 96%
- Human interictal epileptiform discharges are bidirectional traveling waves echoing ictal discharges 96%
- Optogenetic low-frequency stimulation of dentate granule cells prevents seizure generation in experimental epilepsy 95%
Similar papers in this journal
- Distinct changes to hippocampal and medial entorhinal circuits emerge across the progression of cognitive deficits in epilepsy 97%
- Human acute microelectrode array recordings with broad cortical access, single-unit resolution and parallel behavioral monitoring 95%
- RNA-programmable cell type monitoring and manipulation in the human cortex with CellREADR 94%
Similar papers in this journal
- Sensory regulation of absence seizures in a mouse model of Gnb1 encephalopathy 96%
- Context-dependent NMDA receptor dysfunction predicts seizure treatment in mice with human GluN1 variant 96%
- Two-photon voltage imaging of spontaneous activity from multiple neurons reveals network activity in brain tissue 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.