A megatransposon drives the adaptation of Thermoanaerobacter kivui to carbon monoxide
Hocq, R.; Horvath, J.; Stumptner, M.; Thallinger, G. G.; Pfluegl, S.
Show abstract
Acetogens are promising industrial biocatalysts for upgrading syngas, a gas mixture containing CO, H2 and CO2 into fuels and chemicals. However, CO severely inhibits growth of many acetogens, often requiring extensive adaptation to enable efficient CO conversion ("carboxydotrophy"). Here, we adapted the thermophilic acetogen Thermoanaerobacter kivui to use CO as sole carbon and energy source. Isolate CO-1 exhibited extremely rapid growth on CO and syngas (co-utilizing CO, H2 and CO2) in batch and continuous cultures ({micro}max [~] 0.25 h-1). The carboxydotrophic phenotype was attributed to the mobilization of a CO-inducible megatransposon originating from the locus responsible for autotrophy in T. kivui. Transcriptomics illuminated the crucial role maintaining redox balance likely plays during carboxydotrophic growth. These novel insights were exploited to rationally engineer T. kivui to grow on CO. Collectively, our work elucidates a primary mechanism responsible for the acquisition of carboxydotrophy in homoacetogens and showcases how transposons can orchestrate evolution.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Unexpectedly high mutation rate of a deep-sea hyperthermophilic anaerobic archaeon 96%
- Syntrophus Conductive Pili Demonstrate that Common Hydrogen-Donating Syntrophs can have a Direct Electron Transfer Option 95%
- Genetic and phylogenetic analysis of dissimilatory iodate-reducing bacteria identifies potential niches across the world's oceans 95%
Similar papers in this journal
- Establishing Vibrio natriegens as a high-performance host for acetate-based poly-3-hydroxybutyrate production 98%
- Replacing the Calvin cycle with the reductive glycine pathway in Cupriavidus necator 98%
- Awakening of the RuMP cycle for partial methylotrophy in the thermophile Parageobacillus thermoglucosidasius 96%
Similar papers in this journal
- Adaptation of Bacillus thuringiensis to plant colonisation affects differentiation and toxicity 96%
- Longitudinal, Multi-platform Metagenomics Yields a High-quality Genomic Catalog and Guides an In Vitro Model for Cheese Communities 96%
- A salvaging strategy enables stable metabolite provisioning among free-living bacteria 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.