Involvement of 5' and 3' UTRs on SARS-CoV-2 Genome Packaging
Zhang, Z.; Yang, K.; Shao, F.; Shen, W.; Li, P.; Zhang, Y.; Xu, J.; Wang, C.; Xie, D.; Yu, G.; Zhang, J.; Zhao, Z.; Zhang, Y.
Show abstract
The SARS-CoV-2 virus, responsible for the COVID-19 pandemic, packages its large single-stranded RNA genome through a precise yet enigmatic mechanism. A packaging signal (PS) within its genome was proposed to facilitate the assembly of new viral particles. Here in this study, we report the role of the 5 and 3 untranslated regions (UTRs) in PS-mediated genome packaging. Utilizing proximity ligation data, we demonstrate direct interactions between the UTRs and the PS9 element, a key packaging signal within the SARS-CoV-2 genome. Multiple evidence confirmed that the presence of UTRs enhance the packaging efficiency of infectious virus-like particles (iVLPs) and recruits more nucleocapsid (N) protein, suggesting a critical role in genome compaction and packaging. These insights into the regulatory mechanisms of SARS-CoV-2 genome packaging provide novel targets for antiviral therapeutics and contribute to the broader understanding of coronavirus assembly.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Cell culture model system utilizing engineered A549 cells to express high levels of ACE2 and TMPRSS2 for investigating SARS-CoV-2 infection and antivirals 94%
- SARS-CoV-2 cell-to-cell infection is resistant to neutralizing antibodies 94%
- Establishment of a reverse genetic system from a bovine derived Influenza D virus isolate. 94%
Similar papers in this journal
- The impact of clade B lineage 5 MERS coronaviruses spike mutations from 2015 to 2023 on virus entry and replication competence 95%
- Inhibition of Anti-viral Stress Granule Formation by infectious bronchitis virus endoribonuclease nsp15 Ensures Efficient Virus Replication 95%
- Antiviral activity of intracellular nanobodies targeting the influenza virus RNA-polymerase core 95%
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
- Identification of novel wraparound transcripts in JC polyomavirus 95%
- Key mutations on spike protein altering ACE2 receptor utilization and potentially expanding host range of emerging SARS-CoV-2 variants 94%
- The single amino acid change of R516K enables efficient generation of vesicular stomatitis virus-based Crimean-Congo hemorrhagic fever reporter virus 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.