Joint operation of the CO2 concentrating mechanism and photorespiration in green algae during acclimation to limiting CO2
Dao, O.; Bertrand, M.; Alseekh, S.; Veillet, F.; Auroy, P.; Nguyen, P.-C.; Legeret, B.; Epting, V.; Morin, A.; Cuine, S.; Monteil, C.; Mackinder, L. C. M.; Burlacot, A.; Krieger-Liszkay, A.; Weber, A. P. M.; Fernie, A. R.; Peltier, G.; Li-Beisson, Y.
Show abstract
Due to low availability of CO2 in aquatic environment, microalgae have evolved a CO2 concentrating mechanism (CCM). It has long been thought that operation of CCM would suppress photorespiration by increasing the CO2 concentration at the Rubisco active site, but experimental evidence is scarce. To better explore the function of photorespiration in algae, we first characterized a Chlamydomonas reinhardtii mutant defected in low-CO2 inducible 20 (LCI20) and show that LCI20 is a chloroplast-envelope glutamate/malate transporter playing a role in photorespiration. By monitoring growth and glycolate excretion in mutants deficient in either CCM or photorespiration, we conclude that: i. CCM induction does not depend on photorespiration, ii. glycolate excretion protects algal cells from the toxicity of unmetabolized photorespiratory intermediates, iii. photorespiration is active at low CO2 when the CCM is operational. This work provides a foundation for a better understanding of the carbon cycle in the ocean where significant glycolate concentrations have been found.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Impaired photoprotection in Phaeodactylum tricornutum KEA3 mutants reveals the proton regulatory circuit of diatoms light acclimation. 94%
- Mitochondrial respiration is essential for photosynthesis-dependent ATP supply of the plant cytosol 94%
- Thylakoids reorganization enables driving photosynthesis under far-red light in the microalga Nannochloropsis gaditana 94%
Similar papers in this journal
- Modulation of xanthophyll cycle impacts biomass productivity in the marine microalga Nannochloropsis 95%
- The Arabidopsis NRT1/PTR FAMILY Protein NPF7.3/NRT1.5 is an Indole-3-butyric Acid Transporter Involved in Root Gravitropism 94%
- Photoreceptor-induced LHL4 protects photosystem II in Chlamydomonas reinhardtii 94%
Similar papers in this journal
- High Light and High Temperature Reduce Photosynthesis via Different Mechanisms in the C4 Model Setaria viridis 95%
- A deeply conserved protease, acylamino acid-releasing enzyme (AARE), acts in ageing in Physcomitrella and Arabidopsis 94%
- Systems-wide Analysis Revealed Shared and Unique Responses to Moderate and Acute High Temperatures in the Green Alga Chlamydomonas reinhardtii 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.