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Quantitative Comparison of Monomeric StayGold Variants Using Protein Nanocages in Living Cells

Viola, G.; Jacobs, K. A.; Lemiere, J.; Kutys, M. L.; Wittmann, T.

2024-09-17 cell biology Community evaluation
10.1101/2024.09.16.613379 bioRxiv
Show abstract

To standardize comparison of fluorescent proteins and independently determine which monomeric StayGold variant is best for live microscopy, we analyzed fluorescent protein tagged I3-01 peptides that self-assemble into stable sixty subunit dodecahedrons inside live cells. We find mStayGold is 3-fold brighter and 3-fold more photostable compared with EGFP and superior to other monomeric variants in mammalian cytoplasm. In addition, analysis of intracellular nanocage diffusion confirms the monomeric nature of mStayGold.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.