Macrophage-derived RNAseT2 stimulates muscle stem cell fusion via SLK/N-WASP/actin bundling
Weiss-Gayet, M.; Juban, G.; Le Moal, E.; Moretta, A.; Farnetari, C.; Gobet, C.; Guillemaud, J.; Le Bihan, M.-C.; Shoseyov, O.; Adrait, A.; Ternka, K.; Boespflug-Tanguy, O.; Kettwig, M.; Coute, Y.; Mounier, R.; Acquati, F.; Knight, R.; Chazaud, B.
Show abstract
Muscle stem cells (MuSCs) fuse to form myofibers to repair skeletal muscle after injury. Within the regenerative MuSC niche, restorative macrophages stimulate MuSC fusion, although the molecular mechanisms involved are largely unknown. Here, we show that restorative macrophages secrete ribonuclease T2 (RNAseT2) to stimulate MuSC fusion. RNAseT2 entered MuSCs via the mannose receptor and induced the formation of actin bundles in MuSCs, enabling cell/cell fusion. Mechanistically, RNAseT2 bound to Ste20-like kinase (SLK), which itself triggered the phosphorylation-mediated activation of N-WASP, through Paxillin phosphorylation, allowing actin bundling necessary for MuSC fusion. In vivo, overexpressing RNAseT2 in regenerating muscle increased fusion in newly formed myofibers in mouse and zebrafish while macrophages deficient for RNAseT2 gene led to fusion defect and smaller myofibers. This study reveals a new function for the highly conserved RNAseT2 and provides a new molecular mechanism by which restorative macrophages support MuSC fusion during muscle repair.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- In vivo self-renewal and expansion of quiescent stem cells from a non-human primate 96%
- Hedgehog signaling via its ligand DHH acts as cell fate determinant during skeletal muscle regeneration 96%
- Excess PrPC inhibits muscle cell differentiation via miRNA-enhanced liquid-liquid phase separation implicated in myopathy 96%
Similar papers in this journal
Similar papers in this journal
- AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretion 96%
- Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice 94%
- Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury 94%
Similar papers in this journal
- A cell cycle-linked mechanism for the glutamine driven establishment of stem cell fate 95%
- SWELL1-LRRC8 complex regulates skeletal muscle cell size, intracellular signalling, adiposity and glucose metabolism 95%
- Rescue of aged muscle stem cell quiescence defects by AKT inhibition revealed with a 3D biomimetic culture assay 94%
Similar papers in this journal
- Intravital microscopy of satellite cell dynamics and their interaction with myeloid cells during skeletal muscle regeneration 96%
- Immunomodulatory Role of the Stem Cell Circadian Clock in Muscle Repair 96%
- Integrative molecular roadmap for direct conversion of fibroblasts into myocytes and myogenic progenitor cells 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.