MITF Targets in Gastrointestinal Stromal Tumors: Implication in Autophagy and Extracellular Vesicle Secretion
Proano-Perez, E.; Serrano-Candelas, E.; Guerrero, M.; Gomez-Peregrina, D.; Llorens, C.; Soriano, B.; Gamez-Valero, A.; Herrero-Lorenzo, M.; Marti, E.; Serrano, C.; Martin, M.
Show abstract
Previous studies have identified Microphthalmia-associated Transcription Factor (MITF) involvement in regulating Gastrointestinal Stromal Tumors (GIST) growth and cell cycle progression. This study uses Chromatin Immunoprecipitation combined with high-throughput sequencing (ChIP-seq) and RNA sequencing to explore MITF-modulated genes in GIST. Our findings reveal that MITF regulates genes involved in lysosome biogenesis, vesicle generation, autophagy, and mTOR signaling pathways. Comparative transcriptome analysis following MITF silencing in GIST cells shows differential enrichment in mTOR signaling, impacting tumor growth and autophagy. In the context of cancer, the interplay between autophagy and extracellular vesicle release can influence tumor progression and metastasis. We examined MITFs role in autophagy and extracellular vesicle (EV) production in GIST, finding that MITF overexpression increases autophagy, as shown by elevated LC3II levels while silencing MITF disrupts autophagosome and autolysosome formation. Despite no significant changes in EV size or number, MITF silencing notably reduces KIT expression in EV content. KIT secretion in EVs has been linked to GIST metastasis, suggesting that MITF is a crucial target for managing tumor growth and metastasis in GIST.
Matching journals
The top 12 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- ETV2 regulates PARP-1 binding protein to induce ER stress-mediated cell death in tuberin-deficient cells 94%
- ERLIN1/2 scaffolds bridge TMUB1 and RNF170 and restrict cholesterol esterification to regulate the secretory pathway 94%
- An integrated stress response-independent role of GCN2 prevents excessive ribosome biogenesis and mRNA translation 94%
Similar papers in this journal
- Ezrin defines TSC complex activation at endosomal compartments through EGFR-AKT signaling. 94%
- Extracellular ATP drives pancreatic cancer cell invasion via purinergic receptor-integrin interactions 94%
- Proteomic landscape of tunneling nanotubes reveals CD9 and CD81 tetraspanins as key regulators 94%
Similar papers in this journal
- NOX4 regulates TGFβ-induced proliferation and self-renewal in glioblastoma stem cells. 94%
- CRISPR targeting of FOXL2 c.402C>G mutation reduces malignant phenotype in granulosa tumor cells and identifies anti-tumoral compounds 93%
- USP28 enables oncogenic transformation of respiratory cells and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K. 92%
Similar papers in this journal
Similar papers in this journal
- Novel roles of small extracellular vesicles in regulating the quiescence and proliferation of neural stem cells 93%
- In depth exploration of the alternative proteome of Drosophila melanogaster 92%
- NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.