Active EB1 surges promote tubulin influx into the growing outer segments of the bipartite olfactory cilia in Drosophila
Agarwal, R. G.; Iyer, S.; Barbora, A.; Gadgil, Y.; Jana, S.; Ray, K.
Show abstract
Like a photoreceptor cilium, the sensory cilia have a complex bipartite architecture containing 9+0 connecting cilium at the base and a singlet microtubule-supported, highly membranous outer segment, essential for the receptor display. How such diverse cilia morphology and underlying microtubule cytoskeleton develops remains unclear. Here we show that individual olfactory cilium, inside the large basiconic sensilla in developing Drosophila antenna, grows in episodic steps following several pulsatile influxes of tubulin. Each tubulin influx event is preceded by transient elevations of a microtubule-stabilising protein, the End-binding protein 1 (EB1). Additionally, EB1 is found to specifically interact with the tail domain of Drosophila KLP68D, an orthologue of the kinesin-2{beta} motor subunit, in vitro. Finally, the loss of EB1 in olfactory neurons preceding the growth surges reduces the tubulin influx as well as arrests the olfactory cilia assembly and stability. These findings suggest a novel mechanism of bipartite cilia assembly.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Small Interactor of PKD2 (SIP), a novel PKD2-related single-pass transmembrane protein, is required for proteolytic processing and ciliary import of Chlamydomonas PKD2 95%
- Mask, the Drosophila Ankyrin Repeat and KH domain-containing protein, regulates microtubule dynamics 95%
- The FHA domain is essential for the autoinhibition of KIF1A/UNC-104 95%
Similar papers in this journal
- Ancestral role of Pax6 in chordate brain regionalization 94%
- Development of the Pre-Gnathal Segments of the Insect Head Indicates They Are Not Serial Homologues of Trunk Segments 93%
- FOP plays a negative role in ciliogenesis and promotes cell cycle re-entry by facilitating primary cilia disassembly 93%
Similar papers in this journal
- Ataxin-2 is essential for neurodevelopment in Drosophila and is a major regulator of the cytoskeleton. 94%
- The maternal vGluT2 and embryonic mGluR3 signaling relay system controls offspring wing dimorphism in pea aphid 93%
- Novel newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye 93%
Similar papers in this journal
Similar papers in this journal
- The ARF GAPs ELMOD1 and ELMOD3 act at the Golgi and Cilia to Regulate Ciliogenesis and Ciliary Protein Traffic 94%
- Initial ciliary assembly in Chlamydomonas requires Arp2/3 complex-dependent endocytosis 94%
- Proteomic analysis of microtubule inner proteins (MIPs) in Rib72 null Tetrahymena cells reveals functional MIPs 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.