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Epigenetic control of commensal induced Th2 Responses and Intestinal immunopathology

Sangani, K. A.; Parker, M. E.; Anderson, H. D.; Chen, L.; Pandey, S. P.; Pierre, J. F.; Meisel, M.; Riesenfeld, S. J.; Hinterleitner, R.; Jabri, B.

2024-08-30 immunology
10.1101/2024.08.30.610485 bioRxiv
Show abstract

Understanding the initiation of T-helper (Th)-2 immunity is crucial for addressing allergic diseases that have been linked to the commensal microbiota. However, Th2 responses are notably absent from known host-microbiota intestinal immune circuits. Notably, the commensal protist Tritrichomonas induces a transient innate ILC2 circuit rather than a chronic Th2 circuit. Canonical Th2 responses rely on the induction of IL-4 production by innate cells. This study shows that the absence of Tet2, a DNA demethylase, reprograms naive T cells to autonomously produce IL-4 upon T cell receptor stimulation, bypassing the need for IL-4 from innate cells for Th2 differentiation. Loss of this checkpoint induces chronic Th2 responses to Tritrichomonas, associated with IL-25-dependent barrier dysfunction and increased susceptibility to allergic pathology in response to dietary antigens. Sentence SummaryRegulation of cell autonomous IL-4 in T cells is critical to prevent dysregulated Th2 immunity to commensals and predisposition to allergy.

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