A non-canonical CDK, Pho85 regulates the restart of the cell-cycle following stress
Jin, N.; Jin, Y.; Oikawa, Y.; Nakano, A.; Ohsumi, Y.; Weisman, L. S.
Show abstract
Environmental stress induces an arrest of the cell cycle. Thus, release from this arrest is essential for cell survival. The cell-cycle-arrest occurs via the down regulation of the cyclins that drive the main cyclin dependent kinase, CDK1/Cdc28. However, it was not clear how cells escape this potentially fatal arrest. Here we show that prior to the restoration of CDK1/Cdc28 cyclins, a non-canonical CDK, Pho85, initiates a cascade to restart the cell cycle. We demonstrate that following stress, Pho85 phosphorylates the Sch9 kinase, which in turn directly phosphorylates the transcriptional inhibitor Whi5, the yeast analog of RB1/retinoblastoma, and a CDK1 target. This promotes Whi5 translocation from the nucleus, and the release of the stress-induced arrest at G1 phase. In addition, we find that in parallel with Pho85, CDK1/Cdc28 also plays a role in the control of Whi5. Together, these findings provide insights into how cells re-enter the cell cycle during recovery from stress and reveal that a non-canonical CDK and cyclin takes on essential roles and acts via a pathway that functions in parallel with CDK1/Cdc28.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Pak1 kinase controls cell shape through ribonucleoprotein granules 96%
- Growth-dependent signals drive an increase in early G1 cyclin concentration to link cell cycle entry with cell growth 96%
- Mec1-Independent Activation of the Rad53 Checkpoint Kinase Revealed by Quantitative Analysis of Protein Localization Dynamics 96%
Similar papers in this journal
- The TOG protein Stu2 is regulated by acetylation 94%
- The PP2A-like phosphatase Ppg1 mediates assembly of the Far complex to balance gluconeogenic outputs and adapt to glucose depletion 94%
- Fission yeast Rad8/HLTF facilitates Rad52-dependent chromosomal rearrangements through PCNA lysine 107 ubiquitination 94%
Similar papers in this journal
- Genetic requirements for repair of lesions caused by single genomic ribonucleotides in S phase 94%
- Peroxisomal compartmentalization of amino acid biosynthesis reactions imposes an upper limit on compartment size 94%
- Cyclin CLB2 mRNA localization and protein synthesis link cell cycle progression to bud growth 93%
Similar papers in this journal
- The phosphatase Glc7 controls eisosomal response to starvation via posttranslational modification of Pil1 95%
- Decapping complex is essential for functional P-body formation and is buffered by nuclear localization 95%
- Characterization of Pik1 function in fission yeast reveals its conserved role in lipid synthesis and not cytokinesis 95%
Similar papers in this journal
- Stress-dependent inhibition of cell polarity through unbalancing the GEF/GAP regulation of Cdc42 96%
- The GATA transcription factor Gaf1 represses tRNA genes, inhibits growth, and extends chronological lifespan downstream of fission yeast TORC1 95%
- Metabolism of glucose activates TORC1 through multiple mechanisms in Saccharomyces cerevisiae 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.