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Glymphatic clearance is enhanced during sleep

Kroesbergen, E.; Riesselmann, L. V.; Gomolka, R. S.; Pla, V.; Esmail, T.; Stenmo, V. H.; Kovacs, E. R.; Nielsen, E. S.; Goldman, S. A.; Nedergaard, M.; Weikop, P.; Mori, Y.

2024-08-26 neuroscience
10.1101/2024.08.24.609514 bioRxiv
Show abstract

We here revisited the concept that glymphatic clearance is enhanced by sleep and anesthesia. Utilizing dynamic magnetic resonance imaging (MRI), single photon emission computed tomography (SPECT), and fluorescent fiber photometry, we report brain glymphatic clearance is enhanced by both sleep and anesthesia, and sharply suppressed by wakefulness. Another key finding was that less tracer enters the brains of awake animals and that brain clearance across different brain states can only be compared after adjusting for the injected tracer dose.

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