Hydrogen Bonding Magnetic Resonance (HBMR)-based Cyclic Electromagnetic DNA Simulation (CEDS) can affect DNA Hybridization and Conformation

The proton magnetic resonance is commonly used in MRI machines with a strong magnetic field of over 1 T, while this study hypothesized that a weak magnetic field below 0.01 T can induce the proton magnetic resonance in hydrogen bonds of double-stranded DNA (dsDNA). This study found the hydrogen bonding magnetic resonance (HBMR) in dsDNA can modulate the conformations and functions of dsDNAs (1). Decagonal or dodecagonal cyclic electromagnetic DNA simulation (CEDS) was designed to target three-dimensional structures of randomly oriented dsDNAs with about 25% efficiency. This study found that the most effective magnetic exposure times for inducing an electric potential in A-T and G-C base pairs were 280 and 480 msec, respectively. Decagonal CEDS using a target sequence at 20-25 Gauss for 30 min was able to induce sequence-specific hybridization of target short oligo-dsDNAs in 0.005M NaCl solution and their unique conformation in 0.1M NaCl solution. There was a tendency that Pyu oligo-dsDNAs showed more hybridization and unique conformational changes by CEDS using a target sequence than Puy oligo-dsDNAs. This CEDS effect on Pyu ds6(2C2A) increased with CEDS time up to 90 min and gradually decreased to about half (51.8%) of the increase at 240 min resting time. CEDS influenced the oligo-dsDNAs to increase their infrared (IR) absorbance at approximately 3700-2800 cm-1 band compared to the positive and negative controls, which was more dominant in Pyu oligo-dsDNAs than in Puy oligo-dsDNAs. Therefore, it is postulated that HBMR-based CEDS using a weak magnetic field can increase the hybridization potential of oligo-dsDNAs and subsequently lead to unique DNA conformation required for the initiation of various DNA functions. Therefore, it is suggested that decagonal CEDS play a role in regulating the function of target short oligo-dsDNA.