Monitoring DNA double strand break repair in Saccharomyces cerevisiae at high throughput and high resolution
Shalev Ezra, Y.; Saguy, A.; Levin, G.; Weiss, L.; Alalouf, O.; Shechtman, Y.
Show abstract
DNA repair is critical for cellular function and genomic stability across organisms. Yeast mating-type switching serves as an established model for studying DNA break repair and chromatin dynamics. However, real-time tracking of mating-type switching in live cells remains challenging due to resolution limitations of existing techniques. Here, we use high-throughput methods, including three-dimensional imaging, to follow the dynamics of DNA damage and repair and to quantify mating-type switching occurrences at the single live cell level, with unprecedented resolution. We reveal chromatin reconfiguration for both single- and double-strand breaks following switching induction. Our findings provide new observation of the correlation between chromatin folding and single-strand breaks.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Bound2Learn: A Machine Learning Approach for Classification of DNA-Bound Proteins from Single-Molecule Tracking Experiments 97%
- Dps binds and protects DNA in starved Escherichia coli with minimal effect on chromosome accessibility, dynamics and organisation 96%
- RoCi - A Single Step Multi-Copy Integration System Based on Rolling-Circle Replication 95%
Similar papers in this journal
Similar papers in this journal
- Coordinated DNA and Histone Dynamics Drive Accurate Histone H2A.Z Exchange 95%
- Relief of ParB autoinhibition by parS DNA catalysis and ParB recycling by CTP hydrolysis promote bacterial centromere assembly. 94%
- Multiplexing Light-Inducible Recombinases to Control Cell Fate, Boolean Logic, and Cell Patterning in Mammalian Cells 94%
Similar papers in this journal
- CRISPR-Cas12a induced DNA double-strand breaks are repaired by locus-dependent and error-prone pathways in a fungal pathogen 95%
- Engineered cell differentiation and sexual reproduction in probiotic and mating yeasts 95%
- Dynamic cybergenetic control of bacterial co-culturecomposition via optogenetic feedback 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.