AAB-seq: An antigen-specific and affinity-readable high-throughput BCR sequencing method
Hu, M.; Lian, Q.; Cui, X.; Dong, X.; Xin, H.; Shi, W.
Show abstract
B-cell receptor (BCR) sequencing is a powerful antibody discovery tool but current methodology is often inefficient, and lead generation often requires the production and testing of numerous antibody candidates, and it is difficult to provide affinity information for their antibodies at the same time. Here, we introduce AAB-seq (antigen affinity-readable High-throughput BCR sequencing), an efficient antibody screening tool to identify antigen binding affinity of thousands of paired BCRs. It employs fluorophore and DNA barcode-labeled antigen and secondary antibody targeting Ig light chain to label B cells and uses high throughput single cell BCR sequencing and surface protein profiling to obtain the ratio of surface bound antigen to surface BCR in thousands of B-cells. Using AAB-seq, we accurately identified valuable candidate antibodies 1743-3 and 1743-13 from SARS-CoV-2 RBD immunized mouse, providing a basis for further development of SARS-CoV-2 antibody drugs. Thus, AAB-seq allows high throughput identification of antibody sequences paired with antigen affinity, which improves the screening efficiency of functional antibodies and provides an effective solution for the rapid discovery and development of new therapeutic monoclonal antibodies.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Deep repertoire mining uncovers ultra-broad coronavirus neutralizing antibodies targeting multiple epitopes 95%
- Convergent antibody responses to the SARS-CoV-2 spike protein in convalescent and vaccinated individuals 94%
- Mixed IgG Fc immune complexes exhibit blended binding profiles and refine FcR affinity estimates 94%
Similar papers in this journal
- Modular DNA Barcoding of Nanobodies Enables Multiplexed in situ Protein Imaging and High-throughput Biomolecule Detection 94%
- Galectin-9 regulates the threshold of B cell activation and autoimmunity 93%
- Identification of orphan ligand-receptor relationships using a cell-based CRISPRa enrichment screening platform. 93%
Similar papers in this journal
Similar papers in this journal
- scifAI: Explainable machine learning for profiling the immunological synapse and functional characterization of therapeutic antibodies 94%
- Probabilities of HIV-1 bNAb development in healthy and chronically infected individuals 94%
- Single-Cell Profiling of the Antigen-Specific Response to BNT162b2 SARS-CoV-2 RNA Vaccine 94%
Similar papers in this journal
- Elucidating the Characteristics and Clonal Evolutionary Trajectory of Influenza Neuraminidase Broadly Reactive B Cell 94%
- A new universal chimeric-antigen receptor (CAR)- fragment antibody binder (FAB) split system for cancer immunotherapy 94%
- Simultaneous analysis of pMHC binding and reactivity unveils virus-specific CD8 T cell immunity to a concise epitope set 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.