Destablisation of bam transcripts terminates the mitotic phase of Drosophila female germline differentiation
Samuels, T. J.; Torley, E. J.; Naden, E. L.; Blair, P. E.; Hernandez Frometa, F. A.; Karam Teixeira, F.
Show abstract
The tight control of the mitotic phase of differentiation is crucial to prevent tumourigenesis while securing tissue homeostasis. In the Drosophila female germline, differentiation involves precisely four mitotic divisions, and accumulating evidence suggests that bag-of-marbles (bam), the initiator of differentiation, is also involved in controlling the number of divisions. To test this hypothesis, we depleted Bam from differentiating cells and found a reduced number of mitotic divisions. We examined the regulation of Bam using RNA imaging methods and found that the bam 39 UTR conveys instability to the transcript in the 8-cell cyst and early 16-cell cyst. We show that the RNA binding protein, Rbp9, is responsible for timing bam mRNA decay. Rbp9 itself is part of a sequential cascade of RNA binding proteins activated downstream of Bam, and we show that it is regulated through a change in transcription start site, driven by Rbfox1. Altogether, we propose a model in which Bam expression at the dawn of differentiation initiates a series of events that eventually terminates the Bam expression domain.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- C. elegans establishes germline versus soma by balancing inherited histone methylation 97%
- Niche cells regulate primordial germ cell quiescence in response to basement membrane signaling 96%
- VCP acts downstream of tTAFs to downregulate mono-ubiquitinated H2A and promote spermatocyte differentiation in Drosophila 96%
Similar papers in this journal
- Differentiating Drosophila female germ cells initiate Polycomb silencing by altering PRC2 sampling 97%
- Dynamic compartmentalization of the pro-invasive transcription factor NHR-67 reveals a role for Groucho in regulating a proliferative-invasive cellular switch in C. elegans 97%
- Precise temporal control of neuroblast migration through combined regulation and feedback of a Wnt receptor 97%
Similar papers in this journal
Similar papers in this journal
- Simultaneous suppression of ribosome biogenesis and Tor activation by TRIM-NHL proteins promotes terminal differentiation 97%
- Cell cycle exit and stem cell differentiation are coupled through regulation of mitochondrial activity in the Drosophila testis 96%
- Somatic regulation of female germ cell regeneration and development in planarians 96%
Similar papers in this journal
- Characterization of histone inheritance patterns in the Drosophila female germline 96%
- Non-apoptotic caspase activation sustains ovarian somatic stem cell functions by modulating Hedgehog-signalling and autophagy. 96%
- A single-cell transcriptome atlas of the adult muscle precursors uncovers early events in fiber-type divergence in Drosophila 96%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.