Chiral mismatch in collagen-mimetic peptides modulates cell migration through integrin-mediated molecular recognition
Remson, A.; Dellemme, D.; luciano, M.; Surin, M.; Gabriele, S.
Show abstract
Chirality influences essential biological processes such as molecular recognition and self- organization, impacting cell proliferation and differentiation mediated by interactions with the extracellular matrix (ECM). Despite extensive research on cell migration, the role of chirality on matrix-cell interactions has been largely overlooked. To explore this aspect, we engineered culture surfaces coated with natural collagen I or collagen-mimetic peptides (CMPs) with opposite chirality, i.e. with either L- or D-amino acids in their sequences. Here we show that D-surfaces impede epithelial keratocyte spreading, making cells more rounded, less adhesive, and slower. Further investigation through integrin inhibition assays and molecular dynamics simulations revealed that a chiral mismatch destabilizes the triple helix of heterochiral CMPs at the L/D junction. This study underscores the profound impact of ECM chirality on cellular behavior, providing new insights into the relationship between matrix (supra)molecular chirality, integrin-mediated molecular recognition, and cell migration dynamics.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- High-resolution assessment of multidimensional cellular mechanics using label-free refractive-index traction force microscopy 95%
- Combined thermodynamic and time-resolved structural analysis of interactions between AP2 and biomimetic plasma membranes provides insights into clathrin-mediated endocytosis 94%
- Contractility-induced self-organization of smooth muscle cells: from multilayer cell sheets to dynamic three-dimensional clusters 94%
Similar papers in this journal
- Mechanophenotyping of 3D Multicellular Clusters using Displacement Arrays of Rendered Tractions 96%
- Dissecting neurofilament tail sequence-phosphorylation-structure relationships with multicomponent reconstituted protein brushes 94%
- DNA origami demonstrate the unique stimulatory power of single pMHCs as T-cell antigens 94%
Similar papers in this journal
Similar papers in this journal
- Resolving the nanoscale structure of β-sheet assemblies using single-molecule orientation-localization microscopy 95%
- Reconstitution of ultrawide DNA origami pores in liposomes for transmembrane transport of macromolecules 94%
- Time-resolved scanning ion conductance microscopy for three-dimensional tracking of nanoscale cell surface dynamics 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.