Inseparable/IER3IP1 are essential for cytokinesis in Drosophila neuroblast and human cells
Kakade, A. A.; Gupta, S.; Varghese, R.; Adicherla, H.; Nagarkar-Jaiswal, S.
Show abstract
To unveil the molecular players that maintain neural stem cell homeostasis, we conducted a genetic screen in Drosophila and isolated an uncharacterized gene that we named Inseparable (Insep). Insep is the Drosophila homologue of human IER3IP1, a gene associated with Microcephaly, Epilepsy, and Neonatal Diabetes Syndrome (MEDS-1). We show that Insep loss leads to early larval lethality with small brains and these phenotypes can be rescued by expressing IER3IP1 indicating that their biological function is conserved through evolution. The Insep deficient neuroblasts fail to complete cytokinesis and show excessive accumulation of Rab11 vesicles in the cytoplasm. Similarly, IER3IP1 depletion in human cells leads to cytokinesis failure and accumulation of Rab11 vesicles. Insep and IER3IP1 localize to Rab11 vesicles and interact with Rab11. The pathogenic mutations in IER3IP1 perturb its localization to Rab11 vesicles and interaction with Rab11. These results suggest that Insep and IER3IP1 work along with Rab11 and may regulate fusion of Rab11 vesicles to the advancing furrow during cytokinesis.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- The role of Imp and Syp RBPs in precise neuronal elimination by apoptosis through the regulation of TFs 96%
- Rab11 suppresses neuronal stress signaling by localizing Dual leucine zipper kinase to axon terminals for protein turnover. 96%
- delilah, prospero and D-Pax2 constitute a gene regulatory network essential for the development of functional proprioceptors 95%
Similar papers in this journal
- C9orf72-associated arginine-rich dipeptide repeats induce RNA-dependent accumulation of Staufen in nucleus 96%
- Fragile X Premutation rCGG Repeats Impairs Synaptic Growth and Synaptic Transmission at Drosophila larval Neuromuscular Junction 95%
- Alpha-synuclein modulates the positioning of endolysosomes in melanoma cells 94%
Similar papers in this journal
- Nucleolar stress in Drosophila neuroblasts, a model for human ribosomopathies 95%
- A hypomorphic mutation in Pold1 disrupts the coordination of embryo size expansion and morphogenesis during gastrulation 95%
- The Drosophila orthologue of the primary ciliary dyskinesia-associated gene, DNAAF3, is required for axonemal dynein assembly 94%
Similar papers in this journal
- Mask, the Drosophila Ankyrin Repeat and KH domain-containing protein, regulates microtubule dynamics 95%
- The Drosophila Tumour Suppressor Lgl and Vap33 activate the Hippo pathway by a dual mechanism, involving RtGEF/Git/Arf79F and inhibition of the V-ATPase. 95%
- The FHA domain is essential for the autoinhibition of KIF1A/UNC-104 94%
Similar papers in this journal
- Coordination of Pickpocket ion channel delivery and dendrite growth in Drosophila sensory neurons 95%
- α-Phenylalanyl tRNA synthetase competes with Notch signaling through its N-terminal domain 95%
- Mitochondrial fusion regulates proliferation and differentiation in the type II neuroblast lineage in Drosophila 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.