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A high-performance genetically encoded sensor for cellular imaging of PKC activity in vivo

Yahiro, T.; Bayless-Edwards, L.; Jones, J. A.; Ma, L.; Qin, M.; Mao, T.; Zhong, H.

2024-07-23 neuroscience
10.1101/2024.07.19.604387 bioRxiv
Show abstract

We report a genetically encoded fluorescence lifetime sensor for protein kinase C (PKC) activity, named CKAR3, based on Forster resonance energy transfer. CKAR3 exhibits a 10-fold increased dynamic range compared to its parental sensors and enables in vivo imaging of PKC activity during animal behavior. Our results reveal robust PKC activity in a sparse neuronal subset in the motor cortex during locomotion, in part mediated by muscarinic acetylcholine receptors.

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