Previously unknown regulatory role of extracellular RNA on bacterial directional migration
Tetz, V.; Kardava, K.; Vecherkovskaya, M.; Khodadadi-Jamayran, A.; Tsirigos, A.; Tetz, G.
Show abstract
Bacterial directional migration plays a significant role in bacterial adaptation. However, the regulation of this process, particularly in young biofilms, remains unclear. Here, we demonstrated the critical role of extracellular RNA as part of the Universal Receptive System in bacterial directional migration using a multidisciplinary approach, including bacterial culture, biochemistry, and genetics. We found that the destruction or inactivation of extracellular RNA with RNase or RNA-specific antibodies in the presence of the chemoattractant triggered the formation of bacterial "runner cells>> in what we call a "panic state" capable of directional migration. These cells quickly migrated even on the surface of 1.5% agar and formed evolved colonies that were transcriptionally and biochemically different from the ancestral cells. We have also shown that cell-free DNA from blood plasma can act as a potent bacterial chemoattractant. Our data revealed a previously unknown role of bacterial extracellular RNA in the regulation of bacterial migration and have shown that its destruction or inhibition triggered the directional migration of developing and mature biofilms towards the chemoattractant.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Comparative biofilm assays using Enterococcus faecalis OG1RF identify new determinants of biofilm formation 97%
- Capsular polysaccharides cross-regulation modulates Bacteroides thetaiotaomicron biofilm formation 96%
- Pseudomonas aeruginosa leucine aminopeptidase influences early biofilm composition and structure via vesicle-associated anti-biofilm activity 95%
Similar papers in this journal
Similar papers in this journal
- Autotransporters drive biofilm formation and auto-aggregation in the diderm Firmicute Veillonella parvula 96%
- The Diguanylate Cyclase YfiN of Pseudomonas aeruginosa Regulates Biofilm Maintenance in Response to Peroxide 96%
- Differential surface competition and biofilm invasion strategies of Pseudomonas aeruginosa PA14 and PA01 96%
Similar papers in this journal
- Evolution of genotypic and phenotypic diversity in multispecies biofilms 96%
- Biofilm formation displays intrinsic offensive and defensive features of Bacillus cereus 96%
- Cell specialization in cyanobacterial biofilm development revealed by expression of a cell-surface and extracellular matrix protein 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.