Structurally diverse calloses/β-1,3-glucans in plant cell wall microdomains
Amsbury, S.; Marcus, S. E.; Yeshvekar, R.; Barber, J.; German, L.; Ross, J. F.; Lelenaite, I.; de Souza Moraes, T.; Wickramanayake, J.; Klebanovych, A.; Czymmek, K.; Burch-Smith, T. M.; Bayer, E. M.; Willats, W.; Manfield, I. W.; Knox, P.; Benitez-Alfonso, Y.
Show abstract
Cell walls underpin the mechanics of cell growth, intercellular signalling, and defence against pathogenic organisms. {beta}-(1,3)-glucans (also known as callose) are polysaccharides found in plants, fungi, and some bacterial species. In developing plant organs, callose accumulates around intercellular channels (plasmodesmata) controlling cell-to-cell communication. We developed monoclonal antibodies for the detection of {beta}-(1,3)-glucans and using these identified distinct populations of callose differing in size and secondary structure. Callose sub-populations were in proximal but not overlapping cell wall microdomains implying distinct spatial and functional microenvironments. We also unveiled callose interaction with xyloglucan; another plant glycan regulating cell wall functions. This work challenges previous views demonstrating structural heterogeneity in plant callose and supporting interactions between glycans with roles in the regulation of cell wall properties and functions.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Solid-State NMR Analysis of Schizosaccharomyces pombe Reveals Role of α-Amylase Family Enzymes in Cell Wall Structure and Function 95%
- The bacterial virulence factors rhamnolipids and their (R)-3-hydroxyalkanoate precursors activate Arabidopsis innate immunity through two independent mechanisms 94%
- A single-domain expansin-like protein from Gloeophyllum trabeum able to cleave xylan 94%
Similar papers in this journal
- The biosynthesis, degradation, and function of cell wall β-xylosylated xyloglucan mirrors that of arabinoxyloglucan 94%
- A high confidence Physcomitrium patens plasmodesmata proteome by iterative scoring and validation reveals diversification of cell wall proteins during evolution 93%
- CALCIUM-DEPENDENT PROTEIN KINASE32 regulates cellulose biosynthesis through post-translational modification of cellulose synthase 92%
Similar papers in this journal
- The complete {lambda}-carrageenan depolymerization cascade from a marine Pseudoalteromonad revealed by structural analysis of the enzymes. 93%
- The plastoglobule-localized AtABC1K6 is a Mn2+-dependent protein kinase necessary for timely transition to reproductive growth 93%
- Sequential in vitro enzymatic N-glycoprotein modification reveals site-specific rates of glycoenzyme processing 93%
Similar papers in this journal
- Seal milk oligosaccharides rival human milk complexity and exhibit functional dynamics during lactation 93%
- Structure and mechanism of LARGE1 matriglycan polymerase 93%
- Glucomannan engineering highlights the role of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls 93%
Similar papers in this journal
- Eudicot primary cell wall glucomannan is related in synthesis, structure and function to xyloglucan 94%
- Evolutionary variation in MADS-box dimerization affects floral development and protein degradation dynamics in maize 92%
- Thermoprotection by a cell membrane-localized metacaspase in a green alga 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.