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DWARF14 and KARRIKIN INSENSITIVE2 mediate signaling of the apocarotenoid zaxinone in Arabidopsis

Moreno Beltran, J. C.; Shahul Hameed, U. F.; Balakrishna, A.; Ablazov, A.; Liew, K. X.; Jamil, M.; Mi, J.; Alashoor, K.; de Saint Germain, A.; Arold, S. T.; Al-Babili, S.

2024-07-03 plant biology
10.1101/2024.07.03.601835 bioRxiv
Show abstract

The natural growth regulator zaxinone increases the levels of the phytohormones strigolactone (SL) and abscisic acid in Arabidopsis (Arabidopsis thaliana) via unknown mechanisms. We demonstrate that parts of the effects of zaxinone in Arabidopsis depend on the SL receptor DWARF14 (AtD14), the karrikin receptor KARRIKIN INSENSITIVE2 (AtKAI2), and the F-Box protein MORE AXILLARY BRANCHING2 (AtMAX2) that mediates the signaling of SLs and karrikins. Binding assays and co-crystallization revealed zaxinone as an additional ligand of AtD14 and an SL antagonist that interrupts the interaction of AtD14 with AtMAX2. Zaxinone also bound to AtKAI2. These findings unveil a perception mechanism for zaxinone in Arabidopsis and demonstrate the capability of AtD14 and AtKAI2 to bind signaling molecules, other than strigolactones or karrikins, and mediate their transduction.

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