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Integrin-deficient T cell leukemia accumulates in the central nervous system

Lux, S. Y.; Chen, C.; Subhan, B. S.; Chung, H.; Okuniewska, M.; Martin, K. A.; Caslin, A. Y.; Schiavo, J. K.; Vernejoul, J. B.; Froemke, R.; Cammer, M.; Schwab, S. R.

2026-03-08 immunology
10.1101/2024.06.28.601274 bioRxiv
Show abstract

T-cell acute lymphoblastic leukemia (T-ALL) spreads aggressively to the central nervous system (CNS), particularly the leptomeninges. Children with T-ALL are treated with high-dose, CNS-directed chemotherapy, which can cause lasting neurotoxicity and is not always effective. Little is known about how T-ALL enters and persists within the CNS. However, normal T cell migration into the CNS has been extensively studied. Two integrins--VLA-4 and LFA-1--mediate normal T cell entry to the CNS, and VLA-4 blockade effectively treats multiple sclerosis by excluding T cells from the brain. We hypothesized that these integrins would likewise be required for T-ALL CNS entry. Unexpectedly, not only were VLA-4 and LFA-1 dispensable for T-ALL to reach the CNS, integrin-deficient T-ALL accumulated in the CNS compared to control. Mechanistically, integrin loss accelerated T-ALL proliferation in the CNS, suggesting that integrin-mediated interactions may promote quiescence in this space. Integrin blockade synergized with chemotherapy targeting proliferating cells, raising the possibility that combination therapy might be a powerful strategy.

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