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ZFHX3 plays a role in pain and cautious-like behaviours in mice

Nolan, P. M.; Gettings, S. M.; Hillier, R.; Bourbia, N.

2024-06-18 animal behavior and cognition
10.1101/2024.06.18.599525 bioRxiv
Show abstract

A missense mutation in zinc finger homeobox-3 (ZFHX3) gene is known to alter circadian rhythms and metabolism. ZFHX3 is highly expressed in the central nucleus of amygdala and so we investigated whether female and male Zfhx3Sci-/+ mice have impaired pain, fear, anxiety-like, and cautious-like behaviours as these behaviours which are associated with the central nucleus of the amygdala. Using mechanical and thermal sensitivity test, the fear conditioning test and the light-dark box test, we found that female Zfhx3Sci-/+ mice have hypoalgesia to mechanical stimulus while hyperalgesia to heat stimulus. Additionally, both female and male Zfhx3Sci-/+ display reduction of cautious-like behaviour. While neither female and male Zfhx3Sci-/+ mice showed a difference in fear conditioning or anxiety-like behaviour, we have demonstrated for the first time that ZFHX3 is involved in pain and cautious behaviours. HighlightsO_LIFemale and male Zfhx3Sci/+ mice displayed less cautious-like behaviour C_LIO_LIZfhx3Sci/+ female mice had hypoalgesia to noxious mechanical stimulus C_LIO_LIZfhx3Sci/+ female mice had hyperalgesia to noxious heat stimulus C_LI

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