A minimally invasive thrombotic stroke model to study circadian rhythm in awake mice
Marks, K.; Ahn, S. J.; Rai, N.; Anfray, A.; Iadecola, C.; Anrather, J.
Show abstract
Experimental stroke models in rodents are essential for mechanistic studies and therapeutic development. However, these models have several limitations negatively impacting their translational relevance. Here we aimed to develop a minimally invasive thrombotic stroke model through magnetic particle delivery that does not require craniotomy, is amenable to reperfusion therapy, can be combined with in vivo imaging modalities, and can be performed in awake mice. We found that the model results in reproducible cortical infarcts within the middle cerebral artery (MCA) with cytologic and immune changes similar to that observed with more invasive distal MCA occlusion models. Importantly, the injury produced by the model was ameliorated by tissue plasminogen activator (tPA) administration. We also show that MCA occlusion in awake animals results in bigger ischemic lesions independent of day/night cycle. Magnetic particle delivery had no overt effects on physiologic parameters and systemic immune biomarkers. In conclusion, we developed a novel stroke model in mice that fulfills many requirements for modeling human stroke.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Brain-wide continuous functional ultrasound imaging for real-time monitoring of hemodynamics during ischemic stroke 96%
- Assessing post-stroke cognition in pre-clinical models: lessons and recommendations from a multi-center study 95%
- Refined movement analysis in the Staircase test reveals differential motor deficits in mouse models of stroke 95%
Similar papers in this journal
- Blood-brain barrier leakage in the penumbra is associated with infarction on follow-up imaging in acute ischemic stroke 94%
- Low hemoglobin causes hematoma expansion and poor intracerebral hemorrhage outcomes 94%
- Glymphatic system impairment contributes to the formation of brain edema after ischemic stroke 94%
Similar papers in this journal
- Scaling behaviors of deep learning and linear algorithms for the prediction of stroke severity 93%
- A Minimally Invasive, Scalable and Reproducible Neonatal Rat Model of Severe Focal Brain Injury 92%
- Identification of Potential targets for Stroke and Its Subtypes by Integrating Proteomes and Genetics from Human Plasma 90%
Similar papers in this journal
- Astrocyte-secreted chordin-like 1 regulates spine density after ischemic stroke 93%
- Dynamic cerebral autoregulation during and 3 months after endovascular treatment in large-vessel occlusion stroke 92%
- Layer-specific sensory processing impairment in the primary somatosensory cortex after motor cortex infarction 92%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.