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Overdrive is essential for targeted sperm elimination by Segregation Distorter

Ridges, J. T.; Bladen, J.; King, T. D.; Brown, N. C.; Large, C. R. L.; Cooper, J. C.; Jones, A. J.; Loppin, B.; Dubruille, R.; Phadnis, N.

2024-06-06 genetics
10.1101/2024.06.04.597441 bioRxiv
Show abstract

Intra-genomic conflict driven by selfish chromosomes is a powerful force that shapes the evolution of genomes and species. In the male germline, many selfish chromosomes bias transmission in their own favor by eliminating spermatids bearing the competing homologous chromosomes. However, the mechanisms of targeted gamete elimination remain mysterious. Here, we show that Overdrive (Ovd), a gene required for both segregation distortion and male sterility in Drosophila pseudoobscura hybrids, is broadly conserved in Dipteran insects but dispensable for viability and fertility. In D. melanogaster, Ovd is required for targeted Responder spermatid elimination after the histone-to-protamine transition in the classical Segregation Distorter system. We propose that Ovd functions as a general spermatid quality checkpoint that is hijacked by independent selfish chromosomes to eliminate competing gametes.

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