Back

ProtEx: A Retrieval-Augmented Approach for Protein Function Prediction

Shaw, P.; Gurram, B.; Belanger, D.; Gane, A.; Bileschi, M. L.; Colwell, L. J.; Toutanova, K.; Parikh, A. P.

2024-10-17 bioinformatics
10.1101/2024.05.30.596539 bioRxiv
Show abstract

Mapping a protein sequence to its underlying biological function is a critical problem of increasing importance in biology. In this work, we propose ProtEx, a retrieval-augmented approach for protein function prediction that leverages exemplars from a database to improve accuracy and robustness and enable generalization to unseen classes. Our approach relies on a novel multi-sequence pretraining task, and a fine-tuning strategy that effectively conditions predictions on retrieved exemplars. Our method achieves state-of-the-art results across multiple datasets and settings for predicting Enzyme Commission (EC) numbers, Gene Ontology (GO) terms, and Pfam families. Our ablations and analysis highlight the impact of conditioning predictions on exemplar sequences, especially for classes and sequences less well represented in the training data.

Matching journals

The top 6 journals account for 50% of the predicted probability mass.

50% of probability mass above

"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.