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Overlapping role of synaptophysin and synaptogyrin family proteins in determining the small size of synaptic vesicles

Park, D.; Fujise, K.; Wu, Y.; Lujan, R.; Del Olmo-Cabrera, S.; Wesseling, J.; De Camilli, P.

2024-05-31 neuroscience
10.1101/2024.05.29.596401 bioRxiv
Show abstract

Members of the synaptophysin and synaptogyrin family are vesicle proteins with four transmembrane domains. In spite of their abundance in synaptic vesicle (SV) membranes, their role remains elusive and only mild defects at the cellular and organismal level are observed in mice lacking one or more family members. Here, we show that co-expression with synapsin of each of the four brain-enriched members of this family - synaptophysin, synaptoporin, synaptogyrin1 and synaptogyrin3 - in fibroblasts is sufficient to generate clusters of small vesicles in the same size range of SVs. Moreover, mice lacking all these four proteins have larger SVs. We conclude that synaptophysin and synaptogyrin family proteins play an overlapping function in the biogenesis of SVs and in determining their small size.

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