A mitochondria-targeted PPR protein restores cytoplasmic male sterility by post-transcriptional modification of ORF147 in Cajanus cajanifolius
Bhattacharya, J.; Nitnavare, R. B.; Yeshvekar, R.; Reddy, D. S.; Sapara, V.; Reddy, P.; Ramtirtha, Y.; Kalenhalli, Y.; Mathur, P. B.; Reddy, P. S.
Show abstract
Restoration factors (Rfs) belonging to the pentatricopeptide repeat proteins (PPRs) family play an essential role in plant growth and development including their binding to CMS-associated mitochondrial RNAs leading to fertility restoration. The present study identified 22 mitochondrial-specific PPRs in pigeonpea and explored the underlying mechanisms of restoration of fertility in the A4 CMS system through yeast-three hybrid studies. The identified gene was functionally validated through transgenic expression in Arabidopsis model system and obtained conclusive evidence that the identified Rf-PPR was responsible for fertility restoration. The sub-cellular localization studies implied that the identified Rf-PPR is mitochondrial targeting. The study demonstrated that due to the interaction between mitochondrial CMS mRNA and nuclear Rf-PPR protein, post-transcriptional modification occurred, leading to the inability to translate and accumulate cytotoxic CMS protein resulting in fertility restoration. The study specifically looks into the RNA-protein interaction occurring at the nucleo-cytoplasmic level in the A4 cytoplasm of Cajanus cajanifolius. HighlightsThe study identifies the restoration of fertility genes corresponding to the CMS-causing orf147 gene.
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