UPF1 is required for gene expression in mitochondria and paternal mtDNA clearance during spermatogenesis
Wright, M. T.; Singh, A. K.; Dixon, H. L.; Price, E. M.; Connolly, E. C.; di Maio, A.; Barlow, J. P.; Catoni, M.; Fan, Y.; Brogna, S.
Show abstract
UPF1 is a conserved RNA helicase in eukaryotes which is crucial for the correct expression of nuclear genes. This study presents ChIP-seq evidence indicating its RNA-dependent interaction with mtDNA transcription sites in Drosophila S2 cells. Mitochondrial and frequent co-localisation with nucleoids were validated by immunostaining and GFP-tagging of UPF1 in various fly tissues and cell types. Depletion of UPF1, but not other NMD factors, during spermatogenesis, causes severe meiosis and cytokinesis defects, leading to sterility. RNA-seq indicates that these phenotypes could be primarily due to the reduced expression of genes essential for chromosome segregation. Yet also shows that spermatids depleted of UPF1 exhibit a widespread downregulation of mtDNA transcription and, unlike in wild type, fail to eliminate their mtDNA at the elongation stage. Cumulatively, this study indicates a crucial requirement of UPF1 in the expression of both nuclear and mtDNA genes during spermatogenesis.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Importin-9 regulates chromosome segregation and packaging in Drosophila germ cells 96%
- ADAD2 regulates heterochromatin in meiotic and post-meiotic male germ cells via translation of MDC1 96%
- A region of Drosophila SLBP distinct from the histone pre-mRNA binding and processing domains is essential for deposition of histone mRNA in the oocyte 96%
Similar papers in this journal
- Differential modifications of the C-terminal tails of α-tubulin isoforms and their importance for kinesin-based microtubule transport in vivo 97%
- A TOPBP1 Allele Causing Male Infertility Uncouples XY Silencing Dynamics From Sex Body Formation 96%
- A transcription network underlies the dual genomic coordination of mitochondrial biogenesis 96%
Similar papers in this journal
- The Drosophila orthologue of the primary ciliary dyskinesia-associated gene, DNAAF3, is required for axonemal dynein assembly 96%
- Dynamic neurotransmitter specific transcription factor expression profiles during Drosophila development 95%
- Nucleolar stress in Drosophila neuroblasts, a model for human ribosomopathies 95%
Similar papers in this journal
- NuRD independent Mi-2 activity represses ectopic gene expression during neuronal maturation. 95%
- The histone methyltransferase DOT1L regulates chromatin reorganization and gene expression during the postmeiotic differentiation of male germ cells 95%
- SDS-22 stabilizes the PP1 catalytic subunits GSP-1/-2 contributing to polarity establishment in C. elegans embryos 95%
Similar papers in this journal
- VCP acts downstream of tTAFs to downregulate mono-ubiquitinated H2A and promote spermatocyte differentiation in Drosophila 96%
- A sperm-oocyte protein partnership required for egg activation in Caenorhabditis elegans 96%
- The intrinsically disordered protein SPE-18 promotes localized assembly of the major sperm protein in C. elegans spermatocytes 96%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.