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Transit amplifying cells balance growth and differentiation in above-ground meristems

Joossens, J.; Herwegh, D.; Laureyns, R.; Pevernagie, J.; Van Hautegem, T.; Polaris, L.; bhattacharya, S.; Korfhage, C.; Depuydt, T.; Demuynck, K.; Vandepoele, K.; Saeys, Y.; Whipple, C.; Strable, J.; Nelissen, H.

2024-05-05 plant biology
10.1101/2024.05.04.592499 bioRxiv
Show abstract

In both animals and plants, stem cell niches balance between cell-renewal and the generation of progeny cells that differentiate into specialized tissues. The multipotent and highly proliferative transit amplifying cells (TACs) integrate signals from stem cells and their differentiating progeny cells. Here we used spatial transcriptomics mapped to individual cells to illustrate the localization of TACs in maize meristems based on transcriptional gradients. Through genetic interactions and fluctuations in the transcriptional gradients we show that the multiplicative cell divisions are independently controlled from the TAC cell divisions. The dynamic nature of transcriptional variation in response to cell state or environment, together with the potential to improve yield by their modulation highlights the importance of finetuned modulation of networks rather than constitutive perturbations for crop improvement.

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