Validation of plasmonic-based biosensors for rapid and sensitive detection of rabbit hemorrhagic and foot-and-mouth disease viruses in biological samples
Urbinati, C.; di Giovanni, V.; Pezzoni, G.; Capucci, L.; Rusnati, M.
Show abstract
Biosensing technologies and monoclonal antibodies (MAbs) are gaining increasing importance as powerful tools in the field of virology. Surface plasmon resonance (SPR) is an optical biosensing technology already used in virus detection and in the screening of MAbs of diagnostic and therapeutic value. Rabbit haemorrhagic disease virus 2 (RHDV) and foot-and-mouth disease virus (FMDV) are top veterinary issues for whom, the development of novel methods for their detection in biological samples represents a priority with important livestock healthcare and economic implications. With these premises, here we prepared a series of SPR biosensors containing RHDV2 or its 6S subunit immobilized to the surface by different strategies. The biosensors were then used to characterize the binding capacity of a panel of anti-RHDV2 MAbs. From the comparison of the results obtained, the biosensor composed of intact RHDV2 captured with catcher-MAb covalently immobilized to the surface showed the best analytical performances, that were retained also when the same strategy was adopted to prepare a biosensor containing a different virus (namely, FMVD). The results obtained are discussed in view of the exploitation of SPR in the rapid, sensitive and resilient detection of viruses in biological materials and in the screening of antiviral MAbs libraries.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Comparative evaluation of six immunoassays for the detection of antibodies against SARS-CoV-2 93%
- Multicentric Evaluation of a Novel Point of Care Electrochemical ELISA Platform for SARS-CoV-2 Specific IgG and IgM Antibody Assay 93%
- Development and Validation of Novel Cell-free Direct Neutralization Assay for SARS-CoV-2 92%
Similar papers in this journal
- Absolute quantitation of serum antibody reactivity using the Richards growth model for antigen microspot titration 94%
- CovidArray: a microarray-based assay with high sensitivity for the detection of SARS-CoV-2 in nasopharyngeal swabs 93%
- Functionalized TiO2 nanotube-based Electrochemical Biosensor for Rapid Detection of SARS-CoV-2 92%
Similar papers in this journal
- A Paper-based Loop-Mediated Isothermal Amplification (LAMP) Assay for Highly Pathogenic Avian Influenza 92%
- Detection of neutralizing antibodies against SARS-CoV-2 by using a commercial surrogate virus neutralization ELISA: can it substitute the classical neutralization test? 92%
- Portable real-time colorimetric LAMP-device for rapidquantitative detection of nucleic acids in crude samples 92%
Similar papers in this journal
- Development And Performance Evaluation of A Rapid In-House ELISA for Retrospective Serosurveillance of SARS-CoV-2 93%
- Development and Evaluation of Two Rapid Indigenous IgG-ELISA immobilized with ACE-2 Binding Peptides for Detection Neutralizing Antibodies Against SARS-CoV-2 93%
- A novel assessment method for COVID-19 humoral immunity duration using serial measurements in naturally infected and vaccinated subjects 92%
Similar papers in this journal
- A cellular assay for spike/ACE2 fusion: quantification of fusion-inhibitory antibodies after COVID-19 and vaccination 92%
- Evaluation of production lots of a rapid point-of-care lateral flow serological test intended for identification of IgM and IgG against the N-terminal part of the spike protein (S1) of SARS-CoV-2 92%
- Microarray-based detection of antibodies against SARS-CoV-2 proteins, common respiratory viruses and type I interferons 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.