Two distinct Trypanosoma eIF4F complexes co-exist, bind different mRNAs and are regulated during nutritional stress
Papini Gabiatti, B.; Ribeiro Freire, E.; Ferreira da Costa, J.; Galvao Ferrarini, M.; Reichert Assuncao de Matos, T.; Preti, H.; Munhoz da Rocha, I.; Gomes Guimaraes, B.; Kramer, S.; Ivo Tonin Zanchin, N.; Holetz, F. B.
Show abstract
Many eIF4F subunits and PABP paralogues are found in trypanosomes: six eIF4E, five eIF4G, one eIF4A and two PABPs. They are expressed simultaneously and assemble into different complexes, contrasting the situation in metazoans that use distinct complexes in different cell types or developmental stages. Each eIF4F complex has its own proteins, mRNAs and, consequently, a distinct function. We set out to study the function and regulation of the two major eIF4F complexes of Trypanosoma cruzi and identified the associated proteins and mRNAs of eIF4E3 and eIF4E4 in cells in exponential growth and in nutritional stress. Upon stress, eIF4G/eIF4A and PABP remain associated to the eIF4E, but the associations with other 43S pre-initiation factors decrease, indicating that ribosome attachment is impaired. Most eIF4E3-associated mRNAs encode for metabolic proteins, while eIF4E4 associate to mRNAs encoding ribosomal proteins. Interestingly, for both eIF4E3/4, more mRNAs were associated in stressed cells than in non-stressed cells, even though these mRNAs have lower translational efficiencies in stress. In summary, trypanosomes have two co-existing eIF4F complexes involved in translation of distinct mRNA cohorts important for growth. Under stress conditions, both complexes exit translation but remain bound to their mRNA targets.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- DNA replication protein Cdc45 directly interacts with PCNA via its PIP box in Leishmania donovani and the Cdc45 PIP box is essential for cell survival 95%
- Fly stage trypanosomes recycle glucose catabolites and TCA cycle intermediates to stimulate growth in near physiological conditions 95%
- A conserved Guided Entry of Tail-anchored pathway is involved in the trafficking of tail-anchored membrane proteins in Plasmodium falciparum. 95%
Similar papers in this journal
- Mechanisms of translation repression by the EIF4E1-4EIP cap-binding complex of Trypanosoma brucei: potential roles of the NOT complex and a terminal uridylyl transferase 95%
- Proteomic identification of the UDP-GlcNAc : PI α1-6 GlcNAc-transferase subunits of the glycosylphosphatidylinositol biosynthetic pathway of <Trypanosoma brucei>. 95%
- Regulation of transcription elongation anticipates alternative gene expression strategies across the cell cycle 94%
Similar papers in this journal
- Transcriptional shift and metabolic adaptations during Leishmania quiescence using stationary-phase and drug pressure as models 94%
- RNase R, a new virulence determinant of Streptococcus pneumoniae 90%
- Global transcriptional response of Methylorubrum extorquens to formaldehyde stress expands the role of EfgA and is distinct from antibiotic translational inhibition 90%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.