Development of a tightly regulated copper-inducible transient gene expression system in Nicotiana benthamiana incorporating suicide exon and Cre recombinase
Chiang, B.-J.; Lin, K.-Y.; Chen, Y.-F.; Huang, C.-Y.; Goh, F.-J.; Huang, L.-T.; Chen, L.-H.; Wu, C.-H.
Show abstract
Chemical-inducible gene expression systems have been frequently used to regulate gene expression for functional genomics in various plant species. However, a convenient chemical-inducible system that can tightly regulate transgene expression in Nicotiana benthamiana is still missing. In this study, we developed a tightly regulated copper-inducible system that can be used to regulate transgene expression and perform cell death assays in N. benthamiana. We tested several chemical-inducible systems using Agrobacterium-mediated transient expression and found that the copper-inducible system showed the least concerns of leakiness issues. Using the MoClo-based synthetic biology approach, we optimized the design of the copper-inducible system and incorporated the use of the suicide exon HyP5SM/OsL5 and Cre/LoxP as additional regulatory elements to enhance the tightness of the regulation. This new design allowed us to tightly control the hypersensitive cell death induced by several tested NLRs and their matching AVRs, and it can also be easily applied to regulate the expression of other transgenes in transient expression assays. Our findings provide new approaches for both fundamental and translational studies in plant functional genomics.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen production 95%
- Hairy root transformation system as a tool for CRISPR/Cas9-directed genome editing in oilseed rape (Brassica napus) 95%
- AP2/ERF transcription factor NbERF-IX-33 is involved in the regulation of phytoalexin production for the resistance of Nicotiana benthamiana to Phytophthora infestans. 95%
Similar papers in this journal
- Efficient and Versatile Rapeseed Transformation for New Breeding Technologies 95%
- Potato virus X -delivered CRISPR activation programs lead to strong endogenous gene induction and transient metabolic reprogramming in Nicotiana benthamiana. 95%
- Assassination Tango: An NLR/NLR-ID immune receptors pair of rapeseed co-operates inside the nucleus to activate cell death 95%
Similar papers in this journal
- Regulation of CYP94B1 by WRKY33 controls apoplastic barrier formation in the roots leading to salt tolerance 95%
- A theophylline-responsive riboswitch regulates expression of nuclear-encoded genes in Arabidopsis 95%
- Robust survival-based RNAi using in tandem silencing of adenine phosphoribosyltransferase 94%
Similar papers in this journal
- CuBe: a geminivirus-based copper-regulated expression system suitable for post-harvest activation. 97%
- The Arabidopsis immune receptor EFR increases resistance to the bacterial pathogens Xanthomonas and Xylella in transgenic sweet orange 95%
- Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomato 95%
Similar papers in this journal
- A High-throughput Screening Method to Identify Proteins Involved in Unfolded Protein Response Signaling in Plants 96%
- Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants 94%
- A heat-shock inducible system for flexible gene expression in cereals 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.