A Novel Method to Visualize Active Small GTPases Unveils Distinct Sites of Sar1 Activation During Collagen Secretion.
Maeda, M.; Arakawa, M.; Komatsu, Y.; Saito, K.
Show abstract
Small GTPases are essential in various cellular signaling pathways, and detecting their activation within living cells is crucial for understanding cellular processes. Fluorescence resonance energy transfer is widely used to study the interaction between activated small GTPases and their effectors, but it is limited to those with well-defined effectors, excluding Sar1. Here, we present a novel method, SAIYAN (Small GTPase ActIvitY ANalyzing), for detecting the activation of endogenous small GTPases via fluorescent signals utilizing a split mNeonGreen system. We demonstrated Sar1 activation at the endoplasmic reticulum (ER) exit site and successfully detected its activation state in various cellular conditions. Utilizing the SAIYAN system in collagen-secreting cells, we discovered activated Sar1 localized both at ER exit sites and ER-Golgi intermediate compartment (ERGIC) regions. Additionally, impaired collagen secretion led to the confinement of activated Sar1 at the ER exit sites, underscoring the significance of Sar1 activation through the ERGIC in collagen secretion.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- The ER cholesterol sensor SCAP promotes CARTS biogenesis at ER-Golgi contact sites 97%
- Surf4 Promotes Endoplasmic Reticulum Exit of the Lysosomal Prosaposin-Progranulin Complex 97%
- SHIP164 is a Chorein Motif Containing Lipid Transport Protein that Controls Membrane Dynamics and Traffic at the Endosome-Golgi Interface. 97%
Similar papers in this journal
- The SNARE Sec22b regulates phagosome maturation by promoting ORP8-mediated PI(4)P exchange at ER-phagosome contact sites. 97%
- Dynamic Palmitoylation Events Following T-Cell Receptor Signaling 96%
- ER-mitochondria distance is a critical parameter for efficient mitochondrial Ca2+ uptake and oxidative metabolism. 96%
Similar papers in this journal
- Orchestration of SARS-CoV-2 Nsp4 and host-cell ESCRT proteins induces morphological changes of the endoplasmic reticulum 96%
- Recruitment of PI4KIIIbeta to the Golgi by ACBD3 is dependent on an upstream pathway of a SNARE complex and golgins 96%
- Regulation of Blos1 by IRE1 prevents the accumulation of Huntingtin protein aggregates 94%
Similar papers in this journal
- Regulation of Glypican 6-mediated Wnt activation maintains TDP-43 nuclear localization in neurons 95%
- A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells 94%
- Alteration of actin cytoskeletal organisation in fetal akinesia deformation sequence 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.