IMMUNOPHENOTYPING AND FUNCTIONAL ANALYSIS OF NK CELL SUBSETS IN Mycobacterium tuberculosis-INFECTED INDIVIDUALS
Silva, C. S.; Folkesson, E.; Foroogh, F.; Gower, M. S.; Kleberg, L.; Xi, P.; Sarhan, D.; Bruchfeld, J.; Correia-Neves, M.; Källenius, G.; Sundling, C.
Show abstract
Infection with Mycobacterium tuberculosis remains a global health challenge, with diverse clinical outcomes ranging from latent TB (LTB) infection to active TB disease (ATB). We conducted a comprehensive analysis of NK cell subsets and function in individuals with LTB, ATB, and healthy controls to elucidate their potential association with TB pathogenesis. Peripheral blood mononuclear cells (PBMCs) were isolated from Mtb-infected individuals and analyzed by mass cytometry and flow cytometry. We identified distinct NK cell subsets and evaluated their functional responses to stimulation. Our findings revealed comparable frequencies of total and NK cell subsets across LTB, ATB, and controls. Functional assays demonstrated similar degranulation, cytokine production, and proliferation capabilities among NK cell subsets across the three groups. This study provides insights into the heterogeneity of NK cell responses in TB and highlights the need for standardized methodologies with well-characterized cohorts controlling for donor background. Further investigations are warranted to delineate the specific roles of NK cells in TB immunity and pathogenesis.
Matching journals
The top 2 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Inhibition of granuloma triglyceride synthesis imparts control of Mycobacterium tuberculosis through curtailed inflammatory responses 94%
- MR1-restricted MAIT cells from the human lung mucosal surface have distinct phenotypic, functional, and transcriptomic features that are preserved in HIV infection 94%
- Targeting CCRL2 enhances therapeutic outcomes in a tuberculosis mouse model 93%
Similar papers in this journal
- T cells expressing multiple co-inhibitory molecules in acute malaria are not exhausted but exert a suppressive function 95%
- Type I interferons are important co-stimulatory signals during T cell receptor mediated MAIT cell activation 93%
- Vitamin A-treated NK cells reduce IFN-gamma production and support regulatory T cell differentiation 93%
Similar papers in this journal
- Transcriptomic profiling of human effector and regulatory T cell subsets identifies predictive population signatures 92%
- Shared and distinct responses of human and murine alveolar macrophages and monocyte-derived macrophages to Mycobacterium tuberculosis 92%
- The orphan nuclear receptor NR4A3 is dispensable for resident memory CD8+ T cell generation 92%
Similar papers in this journal
- Th2 single-cell heterogeneity and clonal interorgan distribution in helminth-infected mice 95%
- HIV infection alters SARS-CoV-2 responsive immune parameters but not clinical outcomes in COVID-19 disease 93%
- An evolutionary recent IFN-IL-6-CEBP axis is linked to monocyte expansion and tuberculosis severity in humans 93%
Similar papers in this journal
- Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection 95%
- Th22 cells are a major contributor to the mycobacterial CD4+ T cell response and are depleted during HIV infection 94%
- Mycobacteria-specific CD4+IFN-γ+ cell expresses naïve-surface markers and confers superior protection against tuberculosis infection compared to central and effector memory CD4+ T cell subsets 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.