Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM
Rohr, L.; Ehinger, A.; Burmeister, N. G.; Meixner, A. J.; Kemmerling, B.; Harter, K.; zur Oven-Krockhaus, S.
Show abstract
Super-resolution microscopy techniques have revolutionized cell biology by providing insights into the dynamics of single molecules and nanoscale organization within living cells. However, the application of dynamic live-cell methods in plants has been limited by the lack of suitable fluorophores for simultaneous visualization of multiple proteins. To address this challenge, we implemented a two-color sptPALM approach using codon-optimized photoactivatable fluorescent proteins PA-GFP and PATagRFP. Recently, we showed their individual usability in single-color experiments in Nicotiana benthamiana and Arabidopsis thaliana cells. Here, we now demonstrate the suitability of these fluorophores and their combined use for dual-color sptPALM for the simultaneous observation of two different protein fusions in the same plant cell.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Determination of oligomeric states of proteins via dual-color colocalization with single molecule localization microscopy 93%
- A green lifetime biosensor for calcium that remains bright over its full dynamic range 93%
- Engineering paralog-specific PSD-95 synthetic binders as potent and minimally invasive imaging probes 92%
Similar papers in this journal
- High temporal resolution reveals simultaneous plasma membrane recruitment of the TPLATE complex subunits. 94%
- CamelliA-based simultaneous imaging of Ca2+ dynamics in subcellular compartments 92%
- Plasma membrane and cytoplasmic compartmentalization: a dynamic structural framework required for pollen tube tip growth 91%
Similar papers in this journal
- ExoJ: an ImageJ2/Fiji plugin for automated spatiotemporal detection and analysis of exocytosis 93%
- VISION -- an open-source software for automated multi-dimensional image analysis of cellular biophysics 93%
- Light Activated BioID (LAB): an optically activated proximity labeling system to study protein-protein interactions. 92%
Similar papers in this journal
- A comprehensive toolkit for quick and easy visualization of marker proteins, protein-protein interactions and cell morphology in Marchantia polymorpha. 93%
- ANCHOR, a technical approach to monitor single-copy locus localization in planta 93%
- Nanobody-dependent delocalization of endocytic machinery in Arabidopsis root cells dampens their internalization capacity 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.