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Heterogeneity of layer 4 in visual areas of rhesus macaque cortex

Guenther, D. M.; Batiuk, M. Y.; Petukhov, V.; De Oliveira, R.; Wunderle, T.; Buchholz, C. J.; Fries, P.; Khodosevich, K.

2024-03-13 neuroscience
10.1101/2024.03.11.584345 bioRxiv
Show abstract

Non-human primates like rhesus macaques are pivotal models for decoding human visual cortex physiology and disorders. We introduce BrainSPACE, an innovative pipeline for rapid and precise brain tissue banking, to sample visual cortex areas: V1, V2, V4, MT, and TEO. Applying snRNA-seq to V1 and V4 (95,071 nuclei), we uncovered conserved GABAergic neuron profiles but stark area-specific diversity in principal neurons, featuring seven unique layer 4 subtypes in V1 and one in V4. Complementary smFISH validated transcriptional gradients across these areas, aligning with ventral and dorsal stream hierarchies. Gene ontology analyses highlighted plasticity-related pathways in unique layer 4 subtypes, with genes like NTNG1 and NLGN1 linked to neurodevelopmental disorders such as autism and schizophrenia. Our insights bridge molecular architecture to visual processing, offering an interactive atlas for community use. By revealing how layer 4 heterogeneity drives hierarchical specialization, our work advances primate brain mapping and informs therapeutic strategies for vision-related pathologies.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.