pTAC3 and pTAC14 are required for binding of plastid-encoded RNA polymerase to DNA
Wang, J.; Palomar, M.; Min, J.-H.; Wierzbicki, A.
Show abstract
Plastid-encoded RNA polymerase (PEP) is a bacterial-type multisubunit RNA polymerase responsible for the majority of transcription in chloroplasts. PEP consists of four core subunits, which are orthologs of their cyanobacterial counterparts. In Arabidopsis thaliana, PEP associates with 12 PEP-associated proteins (PAPs), which serve as peripheral subunits of the RNA polymerase. The exact contributions of PAPs to PEP function are still poorly understood. We use ptChIP-seq to show that PAP1/pTAC3, a peripheral subunit of PEP, binds to the same genomic loci as RpoB, a core subunit of PEP. The pap1/ptac3 mutant shows a complete loss of RpoB binding to DNA throughout the genome, indicating that PAP1/pTAC3 is necessary for RpoB binding to DNA. A similar loss of RpoB binding to DNA is observed in the pap7/ptac14 mutant, which is defective in another peripheral PEP subunit. We propose that the peripheral subunits of PEP are required for the recruitment of core PEP subunits to DNA. KEY MESSAGEThe peripheral subunits of plastid-encoded RNA polymerase play a crucial role in recruiting the core PEP subunits to DNA in Arabidopsis chloroplasts.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Correlated retrograde and developmental regulons implicate multiple retrograde signals as coordinators of chloroplast development in maize 96%
- Light activates psbA translation in plants by relieving inhibition of translation factor HCF173 by the psbA ORF in cis 96%
- Systematic histone H4 replacement in Arabidopsis thaliana reveals a role for H4R17 in regulating flowering time 95%
Similar papers in this journal
Similar papers in this journal
- Chloroplast quality control pathways are dependent on plastid DNA synthesis and nucleotides provided by cytidine triphosphate synthase two 95%
- The FUSED LEAVES1/ADHERENT1 Regulatory Module Is Required For Maize Cuticle Development And Organ Separation 94%
- BBX16 mediates the repression of seedling photomorphogenesis downstream of the GUN1-GLK1 module during retrograde signaling 94%
Similar papers in this journal
- Duplication of NRAMP3 gene in poplars generated two homologous transporters with distinct functions 95%
- Imprinting and DNA methylation in water lily endosperm: implications for seed evolution 94%
- The expansion and diversification of epigenetic regulatory networks underpins major transitions in the evolution of land plants 93%
Similar papers in this journal
- Neither the availability of D2 nor CP43 limits the biogenesis of PSII in tobacco 94%
- Diphthamide formation in Arabidopsis requires DPH1-interacting DPH2 for light and oxidative stress resistance 94%
- Evolutionary analysis of the LORELEI gene family in angiosperms reveals regulatory subfunctionalization 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.