Molecular regulation of Bna1205ams1 required for male fertility and development of a recessive genic male-sterility system in Brassica napus

Through the comprehensive use of two pollination control systems of cytoplasmic male sterility (CMS) and genic male sterility (GMS), the rapeseed yield was increased by more than 20%. However, more hybrid production systems and detailed mechanisms underlying male sterility are required. Here, we reported a novel two-line hybrid production system of 1205A for GMS and also investigated the underlying mechanism for male sterility. Five co-segregated kompetitive allele specific PCR (KASP) markers were developed and validated, which could be used for transferring the male sterility trait into external B. napus breeding lines and developing further two-line hybrid production systems of GMS. Inheritance studies detected one gene locus of Bna1205ams1 for regulating the male sterility of 1205A. As a potential candidate gene of Bna1205ams1, BnaC03g27700D was fine mapped and narrowed down to a 181.47 kb region on chrC03 and validated by functional analysis. The mutation of BnaC03g27700D in 1205A resulted in large metabolic fluctuations, most of which were involved in aborted tapetal PCD, which could lead to reduced pollen fertility with abnormal pollen exine. The developed new GMS line of 1205AB provided us with the opportunity to identify a new male sterility gene of BnaC03g27700D in B. napus. The study of BnaC03g27700D aims to renew the annotation of the gene and provide new resources for basic research on the genetic control of male sterility.