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An analysis of contractile and protrusive cell behaviours at the superficial surface of the zebrafish neural plate

Araya, C.; Farlie, F.; Moon, L.; Rookyard, C.; Allison, L.; Vizcay-Barrena, G.; Fleck, R.; Tada, M.; Clarke, J.

2024-02-23 developmental biology
10.1101/2024.02.20.581322 bioRxiv
Show abstract

The motive forces underlying convergence and internalisation of the teleost neural plate remain unknown. To help understand this crucial morphogenetic movement we have analysed collective and individual cell behaviours at the superficial surface of the neural plate. Convergence to the midline does not rely on mediolateral cell intercalation, it is characterised by oscillatory contractile behaviours, a punctate distribution of Cdh2 and medially polarised actin-rich protrusions at the surface of the neural plate. Additionally, we characterise and discuss the intimate relationship and dynamic cell surfaces between the motile neural plate and the stationary overlying non-neural enveloping layer.

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