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Cell-autonomous GP130 activation suppresses prostate cancer development via STAT3/ARF/p53-driven senescence and confers an immune-active tumor microenvironment

Sternberg, C.; Limberger, T.; Raigel, M.; Trachtova, K.; Schlederer, M.; Lindner, D.; Kodajova, P.; Yang, J.; Ziegler, R.; Neubauer, H. A.; Dey, S.; Redmer, T.; Stoiber, S.; Hejret, V.; Tichy, B.; Tomberger, M.; Harbusch, N. S.; Tangermann, S.; Oberhuber, M.; Bystry, V.; Persson, J. L.; Pospisilova, S.; Wolf, P.; Sternberg, F.; Hoegler, S.; Lagger, S.; Rose-John, S.; Kenner, L.

2024-02-12 cancer biology
10.1101/2024.02.11.579838 bioRxiv
Show abstract

Prostate cancer ranks as the second most frequently diagnosed cancer in men worldwide. Recent research highlights the crucial roles GP130-mediated signaling pathways play in the development and progression of various cancers, particularly through hyperactivated STAT3 signaling. Here, we find that genetic cell-autonomous activation of the GP130 receptor in prostate epithelial cells triggers active STAT3 signaling and significantly reduces tumor growth in vivo. Mechanistically, genetic activation of GP130 signaling mediates senescence via the STAT3/ARF/p53 axis and anti-tumor immunity via recruitment of cytotoxic T-cells, ultimately impeding tumor progression. In prostate cancer patients, high GP130 mRNA expression levels correlate with better recurrence-free survival, increased senescence signals and a transition from an immune-cold to an immune-hot tumor. Our findings reveal a context-dependent role of GP130/STAT3 in carcinogenesis and a tumor-suppressive function in prostate cancer development. We challenge the prevailing concept of blocking GP130/STAT3 signaling as functional prostate cancer treatment and instead propose cell-autonomous GP130 activation as a novel therapeutic strategy.

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