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RNA G-quadruplex dynamic steers the crosstalk between protein synthesis and energy metabolism

Dumas, L.; Shin, S.; Rigaud, Q.; Cargnello, M.; Hernandez-Suarez, B.; Herviou, P.; Saint-Laurent, N.; Leduc, M.; Le Gall, M.; Monchaud, D.; Dassi, E.; Cammas, A.; Millevoi, S.

2024-01-31 cancer biology
10.1101/2024.01.29.577192 bioRxiv
Show abstract

Cancer cells rely on mitochondria for their bioenergetic supply and macromolecule synthesis. Central to mitochondrial function is the regulation of mitochondrial protein synthesis, which primarily depends on the cytoplasmic translation of nuclear-encoded mitochondrial mRNAs whose protein products are imported into mitochondria. Despite the growing evidence that mitochondrial protein synthesis contributes to the onset and progression of cancer, and can thus offer new opportunities for cancer therapy, knowledge of the underlying molecular mechanisms remains limited. Here, we show that RNA G-quadruplexes (RG4s) regulate mitochondrial function by modulating cytoplasmic mRNA translation of nuclear-encoded mitochondrial proteins. Our data support a model whereby the RG4 folding dynamics, under the control of oncogenic signaling and modulated by small molecule ligands or RG4-binding proteins, modifies mitochondria-localized cytoplasmic protein synthesis. Ultimately, this impairs mitochondrial functions, affecting energy metabolism and consequently cancer cell proliferation.

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