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On the annotation of Paleo- and Mesoproterozoic microfossils as fungi

Kanaparthi, D.; Lampe, M.; Krings, M.; Schue, B. S.; Klingl, A.; Schwille, P.; Lueders, T.

2024-01-29 evolutionary biology
10.1101/2024.01.25.577314 bioRxiv
Show abstract

How old is the crown group Fungi? Inferences from phylogenetic and fossil-based studies provided far-apart age estimates ranging between 0.75 to 2.7 billion years old. One important criterion for interpreting Paleo- and Mesoproterozoic microfossils as Fungi is their uncanny morphological resemblance with extant fungi. Here, we demonstrate that bacteria exposed to environmental conditions similar to the paleoenvironmental settings where these presumed fungi lived can spontaneously transform into their protoplasts. These protoplasts exhibit morphologies corresponding to those of presumed fungal microfossils. These observations, together with microfossil chemical composition, pose a serious challenge to interpreting Paleo- and Mesoproterozoic microfossils as fungi. Based on these results, we reiterate that morphology is not a reliable indicator of the phylogeny of microfossils older than 2.5Ga.

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