MRCK-1 activates non-muscle myosin for outgrowth of a unicellular tube in Caenorhabditis elegans
Popiel, E. M.; Derry, W. B.
Show abstract
The formation and patterning of unicellular biological tubes is essential for metazoan development. It is well established that vascular tubes and neurons use similar guidance cues to direct their development, but the downstream mechanisms that promote the outgrowth of biological tubes are not well characterized. We show that the conserved kinase MRCK-1 and its substrate the regulatory light chain of non-muscle myosin, MLC-4, are required for outgrowth of the unicellular excretory canal in C. elegans. Structure-function analysis of MRCK-1 indicates that the kinase domain, but not the small GTPase-binding CRIB domain, is required for canal outgrowth. Expression of a phosphomimetic form of MLC-4 rescues canal truncations in mrck-1 mutants and shows enrichment at the growing canal tip. Specific depletion of MRCK-1 or MLC-4 in the canal causes severe truncations, with unlumenized projections of the basal membrane. Moreover, our work reveals a novel function for non-muscle myosin downstream of MRCK-1 in excretory canal outgrowth that may be conserved in the development of seamless tubes in other organisms.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- In vivo control of the ezrin/radixin/moesin protein ERM-1 in C. elegans 98%
- Dynamic expression and localization of the LIN-2/7/10 protein scaffolding complex during C. elegans vulval development 98%
- Actin capping protein regulates actomyosin contractility to maintain germline architecture in C. elegans 97%
Similar papers in this journal
- Epidermal PAR-6 and PKC-3 are essential for postembryonic development of Caenorhabditis elegans and control non-centrosomal microtubule organization 97%
- The Nesprin-1/-2 ortholog ANC-1 regulates organelle positioning in C. elegans without its KASH or actin-binding domains 97%
- Gradient-independent Wnt signaling instructs asymmetric neurite pruning in C. elegans 97%
Similar papers in this journal
- Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells 98%
- An extracellular protein regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans 97%
- A C. elegans Zona Pellucida domain protein functions via its ZPc domain 97%
Similar papers in this journal
- Caenorhabditis elegans junctophilin has tissue-specific functions and regulates neurotransmission with extended-synaptotagmin 97%
- Ubiquitin ligases and a processive proteasome facilitate protein clearance during the oocyte-to-embryo transition in Caenorhabditis elegans 96%
- Selective disruption of synaptic BMP signaling by a Smad mutation adjacent to the highly conserved H2 helix 96%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.